In-depth research into rural road crashes

This report was produced under an agreement between Transport SA and the Road Accident Research Unit formed in the late 1990s. Due to various delays in the publication of this report, Transport SA has since become the Department for Transport, Energy and Infrastructure and the Road Accident Research Unit has become the Centre for Automotive Safety Research. The report describes a series of 236 rural road crashes investigated between 1 March 1998 and 29 February 2000 in South Australia. Investigations began with immediate attendance at the scene of the crash. The information collected for each crash included: photographs of the crash scene and vehicles involved, video record of the crash scene and vehicles in selected cases, examination of the road environment, a site plan of the crash scene and vehicle movements in the crash, examination and measurements of the vehicles involved, interviews with crash participants, interviews with witnesses, interviews with police, information on the official police report, information from Coroner’s reports, and injury data for the injured crash participants. The report provides an overall statistical summary of the sample of crashes investigated, followed by a detailed examination of the road infrastructure issues contributing to the crashes. This is done on the basis of crash type, with separate sections concerned with single vehicle crashes, midblock crashes and crashes at intersections. A section is also provided that examines the role of roadside hazards in the crashes.Baldock MRJ, Kloeden CN and McLean A

Exploiting molecular dynamics in Nested Sampling simulations of small peptides

Nested Sampling (NS) is a parameter space sampling algorithm which can be used for sampling the equilibrium thermodynamics of atomistic systems. NS has previously been used to explore the potential energy surface of a coarse-grained protein model and has significantly outperformed parallel tempering when calculating heat capacity curves of Lennard-Jones clusters. The original NS algorithm uses Monte Carlo (MC) moves; however, a variant, Galilean NS, has recently been introduced which allows NS to be incorporated into a molecular dynamics framework, so NS can be used for systems which lack efficient prescribed MC moves. In this work we demonstrate the applicability of Galilean NS to atomistic systems. We present an implementation of Galilean NS using the Amber molecular dynamics package and demonstrate its viability by sampling alanine dipeptide, both in vacuo and implicit solvent. Unlike previous studies of this system, we present the heat capacity curves of alanine dipeptide, whose calculation provides a stringent test for sampling algorithms. We also compare our results with those calculated using replica exchange molecular dynamics (REMD) and find good agreement. We show the computational effort required for accurate heat capacity estimation for small peptides. We also calculate the alanine dipeptide Ramachandran free energy surface for a range of temperatures and use it to compare the results using the latest Amber force field with previous theoretical and experimental results.We acknowledge support from the Leverhulme Trust (Grant F/00 215/BL(NSB, CV and DLW)) and the EPSRC (Grants EP/J020281/1(DLW), EP/J010847/1 (GC) and a Doctoral Training Award (RJB)).This is the final version of the article. It first appeared from Elsevier via http://dx.doi.org/10.1016/j.cpc.2015.12.00

Management and drivers of change of pollinating insects and pollination services. National Pollinator Strategy: for bees and other pollinators in England, Evidence statements and Summary of Evidence

These Evidence Statements provide up-to-date information on what is known (and not known) about the status, values, drivers of change, and responses to management of UK insect pollinators (as was September 2018). This document has been produced to inform the development of England pollinator policy, and provide insight into the evidence that underpins policy decision-making. This document sits alongside a more detailed Summary of Evidence (Annex I) document written by pollinator experts. For information on the development of the statements, and confidence ratings assigned to them, please see section ?Generation of the statements? below. Citations for these statements are contained in the Summary of Evidence document

Targeted modulation of tropoelastin structure and assembly

Tropoelastin, as the monomer unit of elastin, assembles into elastic fibers that impart strength and resilience to elastic tissues. Tropoelastin is also widely used to manufacture versatile materials with specific mechanical and biological properties. The assembly of tropoelastin into elastic fibers or biomaterials is crucially influenced by key submolecular regions and specific residues within these domains. In this work, we identify the functional contributions of two rarely occurring negatively charged residues, glutamate 345 in domain 19 and glutamate 414 in domain 21, in jointly maintaining the native conformation of the tropoelastin hinge, bridge and foot regions. Alanine substitution of E345 and/or E414 variably alters the positioning and interactive accessibility of these regions, as illustrated by nanostructural studies and detected by antibody and cell probes. These structural changes are associated with a lower propensity for monomer coacervation, cross-linking into morphologically and functionally atypical hydrogels, and markedly impaired and abnormal elastic fiber formation. Our work indicates the crucial significance of both E345 and E414 residues in modulating specific local structure and higher-order assembly of human tropoelastin

The Role of Encoding Strategy in Younger and Older Adult Associative Recognition: A Think-Aloud Analysis

Older adults have especially poor recognition memory for word pairs, and recent research suggests this associative deficit manifests primarily in older adults’ higher rates of false alarms compared to younger adults. This could result from older adults either failing to generate meaningful (deep) mediators at study, or failing to benefit from having generated deep mediators at test. Younger and older adults performed a recognition memory task for words and word-pairs. A think-aloud analysis of their spontaneous encoding strategies (e.g., repetition, shallow mediators, and deep mediators) revealed that generation of deep mediators did not differ between younger and older adults, and was associated with high hit rates for items and associates in both age groups. However, generation of deep mediators was inversely related to false alarm rates in younger adults but not older adults. A trial-level analysis of encoding strategies and recognition responses revealed that younger adults benefited from having generated deep mediators when presented with corresponding recombined pairs at test as shown in their lower false alarm rates. In contrast, older adults who generated deep mediators during study (e.g., to blanket-figure) did not benefit from having done so when they encountered the corresponding recombined pairs at test (blanket-summer and district-figure): Their false alarm rates to pairs at test were unrelated to generation of deep mediators at study. These results suggest that many older adults have difficulty retrieving their mediators when presented with recombined pairs at test, older adults’ mediators are not distinct enough to individuate intact pairs from recombined pairs at test, or some combination of both